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Saturday, March 24, 2018
DREAM - Design and development of REAlistic food Models with well-characterised micro- and macro-structure and composition


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Journal of Chromatography A - Elsevier
Available online
Determination of deoxynivalenol, T-2 and HT-2 toxins in a bread model food by liquid chromatography - high resolution - Orbitrap - Mass Spectrometry equipped with a high-energy collision dissociation cell
Monaci L.a, De Angelis E.a, Visconti A.a
aNational Research Council of Italy (ISPA-CNR), Via Amendola 122/O, 70126 Bari.
A sensitive and accurate method employing a single stage high resolution mass spectrometer equipped with a high-energy collision-dissociation cell (HCD) for the simultaneous determination of deoxynivalenol (DON), T-2 and HT-2 toxin in a processed bread model food has been developed.
Two sample pre-treatment routes for the extraction of these mycotoxins were investigated, based on Mycosep column clean-up or QuEChERS-like procedure, respectively. The former approach suffered less from matrix effects and allowed to achieve in bread samples LODs of 7, 12 and 17 ng/g for T-2, HT-2 and DON, respectively, with 0,5 ppm mass accuracy. Two acquisition modes, full scan MS and all ion fragmentation, exploiting the fragmentation features offered by an HCD chamber and integrated within the Orbitrap analyser, were compared for quantitative purposes. The method was applied to investigate the degradation of these mycotoxins during bread processing using a bread model food. Most T-2 hydrolyzed to HT-2 during dough preparation, and about 20-30 % of HT-2 and DON was degraded during bread baking.


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